Dr. Johannes Peham

Dr. Johannes Peham 200x267_Saliva research symposium brisbane 2016Scientist, Austrian Institute of Technology

The biggest challenges for an efficient exploitation of saliva diagnostics at the point of need include the identification and validation of robust biomarkers and the development of sensitive analysis workflows compatible with rapid, automated and miniaturized systems.

Methodology: Two examples will be presented: 1) saliva biomarker discovery by genome wide exosomal microRNA profiling with microarray technology and 2) development of a POC device for oral disease monitoring in saliva based on qPCR.

Results: 1) RNA profiling revealed a significant overlap between serum and saliva-derived exosomal microRNA. 2) Nucleic acid based assays, compatible with POC processing are currently being developed for monitoring of oral pathogens in saliva to enable early detection and personalized therapies for caries and periodontitis.

Conclusion: POC saliva diagnostics requires a comprehensive scientific basis ranging from biomarker discovery to device engineering, which has the great potential to open new fields such as lifestyle monitoring and veterinary applications.

Presentation Title:
Saliva diagnostics: from biomarker discovery to point of care devices
The biggest challenges for an efficient exploitation of saliva diagnostics at the point of need include the identification and validation of robust biomarkers and the development of sensitive analysis workflows compatible with rapid, automated and miniaturized systems.
Methodology: Two examples will be presented: 1) saliva biomarker discovery by genome wide exosomal microRNA profiling with microarray technology and 2) development of a POC device for oral disease monitoring in saliva based on qPCR.
Results: 1) RNA profiling revealed a significant overlap between serum and saliva-derived exosomal microRNA. 2) Nucleic acid based assays, compatible with POC processing are currently being developed for monitoring of oral pathogens in saliva to enable early detection and personalized therapies for caries and periodontitis.
Conclusion: POC saliva diagnostics requires a comprehensive scientific basis ranging from biomarker discovery to device engineering, which has the great potential to open new fields such as lifestyle monitoring and veterinary applications.